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| Registros recuperados: 237 | |
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| Luz María Chacón,J; Lizeth Taylor,C; Carmen Valiente,A; Irene Alvarado,P; Ximena Cortés,B. |
| The availability of a useful tool for simple and timely detection of the most important virulent varieties of Escherichia coli is indispensable. To this end, bacterial DNA pools which had previously been categorized were obtained from isolated colonies as well as selected in terms of utilized phenotype; the pools were assessed by two PCR Multiplex for the detection of virulent E. coli eaeA, bfpA, stx1, stx2, ipaH, ST, LT, and aatA genes, with the 16S gene used as DNA control. The system was validated with 66 fecal samples and 44 wastewater samples. At least one positive isolate was detected by a virulent gene among the 20 that were screened. The analysis of fecal samples from children younger than 6 years of age detected frequencies of 25% LT positive... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Virulence genes; Wastewater; Fecal; Screening system; Escherichia coli. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000400012 |
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| Mattos,José Carlos Pelielo de; Motta,Ellen Serri da; Oliveira,Márcia Betania Nunes de; Dantas,Flávio José da Silva; Araujo,Adriano Caldeira de. |
| Reactive oxygen species (ROS) can induce lesions in different cellular targets, including DNA. Stannous chloride (SnCl2) is a ROS generator, leading to lethality in Escherichia coli (E. coli), with the base excision repair (BER) mechanism playing a role in this process. Many techniques have been developed to detect genotoxicity, as comet assay, in eukaryotic cells, and plasmid DNA agarose gel electrophoresis. In this study, an adaptation of the alkaline gel electrophoresis method was carried out to ascertain the induction of strand breaks by SnCl2 in bacterial DNA, from E. coli BER mutants, and its repair pathway. Results obtained show that SnCl2 was able to induce DNA strand breaks in all strains tested. Moreover, endonuclease IV and exonuclease III play... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Alkaline gel electrophoresis; Escherichia coli; Base excision repair; Stannous chloride; DNA strand breaks; Reactive oxygen species. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700020 |
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| Bueno, L. S.; Ribeiro, J. L.; Poggiani, S. S. C.; Fernandes, M. L.; Souza, S. O.. |
| Os queijos devem ser produzidos com matéria-prima de boa qualidade e submetidos a um eficiente controle em todas as etapas de processamento. A fabricação do queijo envolve vários processos, como pasteurização do leite, coagulação, corte do coágulo, dessoragem, filagem, enformagem, salga, maturação e embalagem. A manipulação do produto, o qual entra em contato com diferentes equipamentos e superfícies, aumenta o risco de contaminação microbiológica. O presente trabalho foi delineado para avaliar a qualidade higiênico-sanitária do processo de fabricação da muçarela produzida em um laticínio da região do Distrito Federal, Brasil, quanto à presença de coliformes totais e termotolerantes. Foram obtidas 15 amostras inerentes às etapas de fabricação: leite cru,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Coliformes totais; Coliformes termotolerantes; Contaminação; Escherichia coli. |
| Ano: 2016 |
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/35025 |
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| Cunha, A. L. F. S.; Paiva, V. N.; Cabrini, C. C.; Vieira, P.; Pinto, M. S.. |
| Os queijos Minas artesanais possuem grande importância econômica, social, ambiental e política para o estado de Minas Gerais, sendo responsáveis pelo sustento direto e indireto de mais de 35 mil famílias. Até o presente momento não existem estudos envolvendo a utilização do fermento natural na fabricação de queijos Minas artesanais. Sabe-se apenas que o mesmo é utilizado de forma indiscriminada em algumas regiões produtoras tradicionais onde alguns produtores utilizam poucos mililitros para cada 100 litros de leite enquanto outros utilizam até um litro para os mesmos 100 litros. Além disso, segundo os produtores, as perdas de produção são atribuídas à qualidade desse fermento natural. O presente trabalho avaliou o efeito da adição de fermento natural sobre... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Segurança Alimentar; Coliformes; Escherichia coli; Staphylococcus aureus. |
| Ano: 2016 |
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/34810 |
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| Astolfi,R.S.; Khouri,D.G.; Brandizzi,L.I.V.; Ávila-Campos,M.J.; Andrade Jr.,H.F. de. |
| Sepsis, the leading cause of death in intensive care units, is associated with overproduction of nitric oxide (NO) due to inducible NO synthase (iNOS), responsible for some of the pathologic changes. Aminoguanidine (AG) is a selective iNOS inhibitor with reported inconsistent actions in sepsis. To investigate the influence of iNOS, we studied models of acute bacterial sepsis using acute challenges with aerobic (Escherichia coli) and anaerobic (Bacteroides fragilis) bacteria in the presence of AG. Six-week-old, 23 g, male and female BALB/c and C57Bl/6j mice, in equal proportions, were inoculated (ip) with bacteria in groups of 4 animals for each dose and each experiment in the absence or presence of AG (50 mg/kg, ip, starting 24 h before challenge and daily... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Nitric oxide; Sepsis; Experimental model; Bacterial infections; Escherichia coli; Bacteroides fragilis. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000300006 |
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| Guidoni,Eliana Biondi Medeiros; Berezin,Eitan N.; Nigro,Stanley; Santiago,Nataly A; Benini,Vanda; Toporovski,Julio. |
| Knowledge about antimicrobial resistance patterns of the etiological agents of urinary tract infections (UTIs) is essential for appropriate therapy. Urinary isolates from symptomatic UTI cases attended at Santa Casa University Hospital of São Paulo from August 1986 to December 1989 and August 2004 to December 2005 were identified by conventional methods. Antimicrobial resistance testing was performed by Kirby Bauer's disc diffusion method. Among the 257 children, E. coli was found in 77%. A high prevalence of resistance was observed against ampicillin and TMP/SMX (55% and 51%). The antibiotic resistance rates for E. coli were: nitrofurantoin (6%), nalidixic acid (14%), 1st generation cephalosporin (13%), 3rd generation cephalosporins (5%), aminoglycosides... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Urinary tract infection; Pediatrics urinary tract infection; Bacterial resistance; Escherichia coli. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000400013 |
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| Camargo,L.R.P.; Suffredini,I.B.. |
| Escherichia coli é uma bactéria presente no trato intestinal de animais de sangue quente. Entretanto, a perda do equilíbrio em sua relação com o hospedeiro pode causar doenças que levam a perdas econômicas, quando se trata de animais de produção, e perdas incalculáveis, quando se trata de animais silvestres. O presente estudo testou diversos extratos aquosos e orgânicos, obtidos de plantas da Amazônia brasileira, contra Escherichia coli (ATCC 25922). Usando o ensaio de disco difusão em ágar Mueller-Hinton (DDA), foram testados 1.791 extratos quanto à sua atividade antibacteriana. Os extratos que mostraram halo de inibição de crescimento bacteriano foram testados no modelo da microdiluição em caldo (MDBC) contra uma suspensão bacteriana preparada na escala... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Escherichia coli; Plant extracts; Pathogenicity. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352014000200041 |
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| Indu,M.N.; Hatha,A.A.M.; Abirosh,C.; Harsha,U.; Vivekanandan,G.. |
| Antibacterial activity of extracts of Allium sativum (garlic), Myristica fragrans (nutmeg), Zingiber officinale (ginger), Allium cepa (onion) and Piper nigrum (pepper) has been evaluated against 20 different serogroups of Escherichia coli, 8 serotypes of Salmonella, Listeria monocytogenes and Aeromonas hydrophila. Garlic extract showed excellent antibacterial activity against all the test organisms, except L. monocytogenes. Nutmeg showed good anti-listerial activity, although activity against E. coli and Salmonella were serotype dependent. Both garlic and nutmeg extracts were effective against A. hydrophila. Extracts of ginger showed inhibitory activity against two serogroups of E. coli: as O8 (enterotoxigenic E. coli) and O88 only. Extracts of onion and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Spices extract; Antibacterial activity; Escherichia coli; Salmonella; Listeria monocytogenes; Aeromonas hydrophila. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000200011 |
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| Lourenço,TC; Mendonça,EP; Nalevaiko,PC; Melo,RT; Silva,PL; Rossi,DA. |
| The aim of this study was to evaluate the efficacy of turmeric (Curcuma longa), also known in Brazil as saffron, on the reduction of Staphylococcus aureus and Escherichia coli counts in chicken meat. Forty breast meat samples were divided in two groups (A and B). In group A, 10³-10(4)E. coli (ATCC 25922) cells were inoculated and group B samples were inoculated with 10(4)-10(5)S. aureus (ATCC 9801) cells, after which each group was divided in three samples. The first sample was analyzed immediately after inoculation. The second sample (control group) was stored at 4 ºC for 48 hours and turmeric at 1% (w/w) was added to the third sample, which was homogenized and then stored under the same conditions as the second sample. E. coli and S. aureus were... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Turmeric; Bacterial growth; Escherichia coli; Staphylococcus aureus. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2013000200002 |
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| Melo,Daniela Benevides; Menezes,Ana Paula de Oliveira; Reis,Joice Neves; Guimarães,Alaíse Gil. |
| Antibiotic resistance has increased in recent years, raising the concern of public health authorities. We conducted a study of Escherichia coli isolates obtained from human and food samples to assess the prevalence of antimicrobial resistance and to determine the genotype and clonal relationship of 84 E. coli isolates (48 from humans and 36 from foods). An antimicrobial susceptibility test was performed using the disk diffusion method. Virulence factors were evaluated by multiplex PCR, and the clonal relationship among the resistant isolates was studied by Pulsed Field Gel Electrophoresis (PFGE). All isolates were susceptible to ceftriaxone. Overall, 26%, 20.2%, 15.4% and 6% of the isolates were resistant to tetracycline, ampicillin,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Escherichia coli; Antimicrobial resistance; Food contamination; Genetic diversity. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000401165 |
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| Carvalho,Daiane; Finkler,Fabrine; Grassotti,Tiela Trapp; Kunert Filho,Hiran Castagnino; Lima,Francisco Esmaile de Sales; Soares,Beatriz Dugaich; Rossato,Juliana Marzari; Cunha,Augusto César da; Brito,Kelly Cristina Tagliari de; Brito,Benito Guimarães de. |
| The study aimed to evaluate the antimicrobial susceptibility of 109 samples of Escherichia coli (E. coli) of environmental origin and to characterize these isolates according to the degree of pathogenicity in vivo, verifying a possible relationship between this variable and susceptibility to the active principles tested. The isolates were subjected to disc diffusion test to 14 antibiotics. From 16.5% to 90% of the samples were sensitive; 1 - 28.5% showed intermediate degree of susceptibility and between 9 to 78% of E. coli analyzed were resistant. The highest resistance percentages were seen in the class of quinolones and tetracyclines (>75%), and for sensitivity in the class of amphenicols (68.8%). By inoculating 1- day - old chicks, the isolates... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Escherichia coli; Resistance; Antimicrobials; Pathogenicity; Poultry.. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015000701249 |
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| Registros recuperados: 237 | |
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